B-1009: Aquatic Veterinary Bacterial Collection, Culture and Identification Techniques

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This presentation will cover culture techniques and identification of bacteria from fish and other aquatic animals. Topics covered include collection of samples, collection and transport with an emphasis for remote areas; culture requirements for bacteria from freshwater, brackish and marine sources including use of NaCl, sea salts and incubation temperature; the advantages and disadvantages of different bacterial identification systems including conventional biochemical identification methods, use of the API systems and Vitek from Biomerieux, Biolog, and MALDI-TOF (matrix-assisted laser desorption ionisation time of flight mass spectrometry. Molecular techniques for identification of particular bacteria will be discussed briefly.